Plasmid_Backbone

Part:BBa_K606069:Design

Designed by: Babak Nichabouri   Group: iGEM11_Paris_Bettencourt   (2011-10-28)


pDCPKO - Integrative plasmid for B. subtilis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal suffix found at 1551
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1552
    Illegal PstI site found at 1566
    Illegal NotI site found at 7
    Illegal NotI site found at 1559
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Illegal suffix found at 1552
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1552
    Illegal PstI site found at 1566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3965
    Illegal SapI.rc site found at 5047


Design Notes

Addition of AmyE 5' and 3'


Source

PBGSC6

References